WORK FLOW FOR MULTIPLEXING
simple method
MIXTURE PREPARATION
Hblock mixture
& sample
LOADING IN CONTAINER
96-well plate
or micro chamber chip
REACTION
INCUBATION
with vortex
MEASUREMENT & ANALYSIS
with FS100
HOW DOES WORK
Functionalized Hblock mixture preparation to Analysis
STEP 1. LIGAND IMMOBILIZATION
Each biotinylated ligand molecule is immobilized on each encoded particle via streptavidin-biotin interaction. you know, streptavidin-biotin interaction is very strong. So this pair of molecules is used in many bio-assay.

STEP 2. LIGAND IMMOBILIZED HBLOCK MIXTURE READY FOR USE
The third step is to mix the immobilized Hblocks in one tube with each ligand.
Finally, you will be able to store the Hblock blend tube, and use it out after each experiment.
If you complete the third phase, you can skip to the third level until the Hblock mixture is exhausted.

STEP 3. INCUBATION FOR LABELING MATERIAL-ANALYTE-LIGAND INTERACTION
At this stage, ligand and analyte bind first and labeling material binds second.
The turn around time will vary depending on the purpose of the test and the concentration level of the analyte, but usually takes 1 to 2 hours.

STEP 4. DECODING & READING
Insert the container into the device.
And when the customer conducts the analysis, it will automatically report the result after a few minutes.
While the customer presses the Analyze button and take a rest, the FS100 will quickly and accurately perform decoding and reading according to predefined our algorithms. That's all.

ANSWER
You can do screening & quantification of multi-analytes using Maximultix. First, you can determine the positive & negative value for the reference value for a number of analytes in one well.
